Corn is one of the strategic food commodities in Indonesia which is currently widely used as animal feed ingredient. But the average productivity of national corn is still low. This study aims to determine the dose of herbicide ammonium glufosinate 200 g/L which can maintain the growth potential and yield of corn plants. The experiment was carried out at the Ciparanje Experimental Garden of the Faculty of Agriculture, Padjadjaran University, Jatinangor, Sumedang Regency, West Java, with altitude of 750 meters above sea level in November 2017 to March 2018. The experimental method used was a randomized block design consisting of seven treatments which were repeated four times. The treatments tested consisted of: manual weeding, application of different doses of ammonium glufosinate (450, 600, 750, 900, and 1050 g/ha) and unweeded check. The results of the experiment showed that application of ammonium glufosinate 200 g/L herbicide is able to suppress weed growth in corn cultivation. The application of ammonium glufosinate 200 g/L herbicide starting from a dose of 600 g/ha is effective in suppressing weed growth and suppressing the loss of yield of corn due to the presence of weeds.