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Document Type : Original Article


Mycology Research Laboratory, Department of Biological Science, Rani Durgawati University, Jabalpur, Madhya Pradesh, INDIA



Parthenium hysterophorus weeds are one of the major problems in almost all types of agricultural lands, forest lands, pastures, farmlands and disturbed land including roadsides. It is also responsible for health problems in human and animals besides loss to agriculture and ecosystems. It causes allergic respiratory problems, contact dermatitis and mutagenicity. The main strategy of Parthenium control is the use of chemical herbicides. The increasing side effects of chemical herbicide and growing resistance against them in weeds have attracted the attention of researchers to search for some novel herbicidal compounds from natural sources. A significant barrier in the mycoherbicide metabolites production is the development of an economically viable fermentation process. The production of these compounds is largely affected by certain parameters like pH, temperature, incubation days and media constituents etc. Adjustment of these parameters up to optimum level leads to maximum production of mycoherbicidal compounds. Therefore, the purpose of this study was to standardize physiochemical conditions (Nutrient media, pH, Temperature, and Incubation periods) for maximum production of mycoherbicidal metabolites from phytopathogenic fungi Fusarium sp. FGCCW#16 isolated from Parthenium hysterophorus weed. Fusarium sp FGCCW#16 grew well and produced optimum mycoherbicidal metabolites in Richard’s broth medium at 25 to 28°C on the 21 studay of the incubation. The optimal growth, however, was obtained at pH 6. The results of this investigation indicated that cultural conditions like culture media as well as physical factors such as temperature, pH and incubation period greatly affected the growth and production of mycoherbicidal metabolites by Fusarium sp FGCCW#16.


Alberts J.F, Gelderblom W.C.A, Thiel P.G, Marasas W.F.O, Vanschalkwyk D.J, Behrend Y. 1990. Effects of Temperature and Incubation Period on Production of Fumonisin B1 by Fusarium moniliforme. Appl Environ Microbiol. 56: 1729-1733.
Ash G.J. 2010. The science, art and business of successful bioherbicides. Biol Cont. 52: 230-240.
Betina A. 1989. Mycotoxins- Chemical, Biological and Environmental Aspects. Elsevier, Amsterdam. 
Bhattacharyya P.N, Jha D.K. 2011. Optimization of cultural conditions affecting growth and improved bioactive metabolite production by a subsurface Aspergillus strain TSF 146. Int J Appl Biol Pharmaceut Technol. 2: 133-143.
Blackburn F, Hayes W.A. 1966. Studies on the nutrition of Arthrobotrys oligospora Fres and A. robusta Dudd I. The saprophytic phase. Ann. Appl. Biol. 58: 43-50.
Boonyapranai K.B, Tungpradit R, Lhieochaiphant S, Phutrakul S. 2008. Optimization of Submerged Culture for the Production of Naphthoquinones Pigment by Fusarium verticillioides. Chiang Mai J. Sci. 35: 457-466.
Boyetchko S.M, Rosskopf E.N, Caesar A.J, Charudattan R. 2002. Biological weed control with pathogens: search for candidates to applications. In: Khachatourians GG, Arora DK eds. Applied Mycology and Biotechnology, Vol. 2. Agriculture and Food Production. The Netherlands: Elsevier Science B.V. pp 239–27
Brun T, Rabuske J.E, Todero I, Almeida T.C, Daniel Junior J.J, Ariotti G, Confortin T, Arnemann J.A, Kuhn R.C, Guedes J.V.C, Mazutti M.A. 2016. Production of bioherbicide by Phoma sp. in a stirred-tank bioreactor. Biotech. 6: 230.
Charudattan R. 2001. Biological control of weeds by means of plant pathogens: Significance for integrated weed management in modern agro-ecology. Biocontrol. 46: 229-260
Churchill B.W. 1982. Mass production of microorganisms for biological control. In: Biological control of weeds with plant pathogens (eds. Charudattan and Walker H.L.). John Wiley and sons, New York, pp. 139-156.
Cordeau S, Triolet M, Wayman S, Steinberg C, Guillemin J.P. 2016. Bioherbicides: dead in the water? A review of the existing products for integrated weed management. Crop Prot. 87: 44-49.
Dayan F.E, Duke S.O. 2014. Natural compounds as next-generation herbicides. Plant Physiol. 166: 1090-1105
Gaur R.B, Agnihotri J.P. 1982. Toxic metabolites of Fusarium solani in relation to onion root rot. Ind J. Mycol. Pl. Pathol. 12: 6-9.
Gbolagade J.S. 2006. The effect of different nutrient sources on biomass production of Lepiota procera in submerged liquid cultures. Afr. J. Biotechnol. 5: 1246-1249.
Gunasekaran S, Poorniammal R. 2008. Optimization of fermentation conditions for red pigment production from Penicillium sp. under submerged cultivation. Afr. J. Biotechnol. 7: 1894-1898.
Harley J.L. 1934. Some critical experiments upon culture methods used for fungi. New Phytol. 33: 372-385.
Ismaiel A, Ahmed E.S, Asmaa A, Mahmoud A. 2010. Some optimal culture conditions for production of Cyclosporine A by Fusarium roseum Proceeding of Fifth Scientific Environmental Conference, Zagazig Uni., Egypt. 21-35.
Jyotsnamayee S, Gupta N. 2010. Nutritional factors affecting the antifungal activity of Penicillium stecki of mangrove origin. Afr J. Microbiol Res. 4: 126-135.
Keller N.P, Turner G, Bennett J.W. 2005. Fungal secondary metabolism - from biochemistry to genomics. Nat Rev Microbiol. 3: 937-947.
Khan I.A, Alam S.S, Jabbar A. 2001. Standardization of medium for the Production of Maximum Phytoxic activity by Fusarium oxysporum f. Sp. ciceris. Paki J. Biol Sci. 4: 1374-1376.
Lakpale N, Kumar R, Khare N. 1996. Studies on the toxin production by Rhizoctonia solani causing sheath of rice. Ind. J. Mycol. Plant. Pathol. 26: 263-265.
Lee H, Song J.H, Ahn C.G, Shin G.P, Lee C. 2008. Statistical optimization of growth medium for the production of the entomopathogenic and phytotoxic cyclic depsipeptide beauvericin from Fusarium oxysporum KFCC 11363P. J. Microbiol. Biotechnol. 18: 138-144.
Lilly V.G, Barnett H.L. 1951 Physiology of fungi. Mc Graw Hill Book Co. Inc. pp. 464.
Liu X.Z, Chen S.Y. 2002. Nutritional requirements of the nematophagous fungus Hirsutella rhossilensis. Biocontrol Sci Technol. 12: 381-393.
Liu X.Z, Li S.D. 2004 Fungi secondary metabolites in biological control of crop pests. In:  Handbook of Industrial Mycology (ed Z.Q. An) Marcel Dekker, New York, pp.723-744.
Mathan S, Subramanian V, Nagamony S. 2013. Optimization and antimicrobial metabolite production from endophytic fungi Aspergillus terreus KC 582297. Eur J Exp Biol. 3: 138-144.
Merlin J.N, Nimal I.V.S, Christhuda S, Praveen K.P, Agastian P. 2013. Optimization of growth and bioactive metabolite production: Fusarium solani, Asian J Pharm Clin Res. 6: 98-103.
Pandey A.K, Singh A.K, Quereshi S, Agrawal D. 2004. Herbicidal activities of secondary metabolites of Streptomyces sp. against Hyptis suaveolens. J. Basic. Appl. Mycol. 3: 95-97.
Pandey A.K, Singh A.K, Quereshi S, Pandey C. 2005. Herbicidal activities of secondary metabolites of Aspergillus spp. against Lantana camara. J. Basic. Appl. Mycol. 4: 65-67.
Parmar P, Ojha V.P, Subramanian R.B. 2010. Optimization of Fusaric acid production by Fusarium oxysporum f. sp. lycopersici using response surface methodology. Ind J. Sci. Technol. 3: 411-416.
Singh A.K. 2007. Isolation and characterization of Herbicidal compounds from some selected fungi. Ph D Thesis. R D University Jabalpur. MP India.
Stanbury P.F, Whitaker A, Hall S.J. 1995. Media for industrial fermentations. In: Principles of fermentation technology (eds. Stanbury P.F, Whitaker A, Hall S.J.) Elsevier Science Ltd. Pergamon Oxford, New York, Tokyo pp. 93-122.
Strobel G.A, Daisy B. 2003. Bioprospecting for microbial endophytes and their natural products. 2003. Microbial Mol Biol Rev. 67: 491-502.
Tanaka T, Hanato K, Watanabe M, Abbas H.K. 1996. Isolation, purification and identification of 2,5-anhydro-D-glucitol as a phytotoxin from Fusarium solani. J. Nat.Toxins. 5: 317-329.
Thakur D.B, Bora T.C, Bordoloi G.N, Mazumdar S. 2009. Influence of nutrition and culturing conditions for optimum growth and antimicrobial metabolite production by Streptomyces sp. J Med Mycol. 19: 161-167.