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Document Type : Original Article


1 Botany Department, Rashtriya Snatkottar Mahavidyalaya, Jaunpur 222001 (Uttar Pradesh) India

2 Department of Biotechnology, Central University of Rajasthan, NH-8, Bandar Sindri, Dist-Ajmer-305817 (Rajasthan) India

3 Department of Biotechnology, Junagadh Agricultural University, Junagadh 362 001, (Gujarat) India

4 CIF Division, National Botanical Research Institute (CSIR) PO Box No 436, Rana Pratap Marg, Lucknow - 226001 (Uttar Pradesh) India

5 Central Institute of Medicinal and Aromatic Plants (Council of Scientific and Industrial Research), Lucknow-226015 (Uttar Pradesh) India

6 Department of Biosciences and Bioinformatics, Khallikote University, Berhampur 760001 (Orissa) India



Parthenium hysterophorus L. is a notorious weed, which significantly reduce yield and quality of crops and causes several problems to human health. In, present study an attempt was made to understand the economic value, and survival nature of Parthenium through deep transcriptome analysis. Transcriptome analysis of leaf and root tissue of P. hysterophorus had resulted 7,832,143 reads in case of leaves, and 9,646,830 reads in case of roots sample with longest read length of 300 and 298 nucleotides, respectively. A total of 35,719 contigs were produced with an average length of 548bp after an assembly in all two samples. The Blastn of the above generated contigs with 61,901 sequences of P. argentatum resulted in the identification of 25,947 novel contigs specific to P. hysterophorus. The Kyoto Encyclopedia of Genes and Genomes pathway based analysis showed the expression of genes associated with pathways pertaining to biosynthesis of Glucosinolate, Amino acids, and Aminobenzoate degradation etc. The expression pattern of genes like Artemisinic aldehyde Delta (11(13)) reductase, Codeine O-demethylase, Taraxerol synthase, and Curculin-2 related to biosynthetic of therapeutic importance pathways was also evidenced. Further, the heavy metal accumulator property of P. hysterophorus was also studied. Expression analysis of heavy metal transporters such as ferrous ion transport protein B, and zinc transporter in roots was also validated with its heavy metal transport activity. This investigation provides new insights for functional studies of P. hysterophorus genes involved in biosynthesis of therapeutically important secondary metabolites, and other possible uses such as raw material for rubber industry.


Aly R, Dubey N.K, Yahyaa M, Abu-Nassar J, Ibdah M. 2014. Gene silencing of CCD7 and CCD8 in Phelipanche aegyptiaca by tobacco rattle virus system retarded the parasite development on the host. Plant signaling & behavior. 9: e29376.
Batish D.R, Singh H.P, Kohli R.K, Saxena D.B, Kaur S. 2002. Allelopathic effects of parthenin against two weedy species, Avena fatua and Bidens pilosa. Environ exp bot. 47: 149-55.
Bhargava A, Carmona F.F, Bhargava M, Srivastava S. 2012. Approaches for enhanced phytoextraction of heavy metals. J environ manag. 105: 103-120.
Bhoyar M.G, Gavkare O.J. 1970. Possible uses of parthenium-An agricultural waste. I Control Pollution. 30: 233-236.
Briat J.F. 1996. Roles of ferritin in plants. J Plant Nut. 19: 1331-42.
Chow K.S, Mat-Isa M.N, Bahari A, Ghazali A.K, Alias H, Mohd.-Zainuddin Z, Hoh C.C, Wan K.L. 2011. Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex. J exp bot. 63: 1863-71.
Domínguez X.A, Sierra A. 1970. Isolation of a new diterpene alcohol and parthenin from Parthenium hysterophorus. Planta medica. 18: 275-7.
Dubey N.K, Yadav P, Gupta N, Gupta K, Panigrahi J, Gupta A.K. 2019. Suppression of seed setting and viability in phytoplasma-infected Parthenium weed in nature through differential gene expression. Indian J Weed Sci. 51:188-97.
Essiett A.A, Israel U, Uwak S.O, Udoimuk A.B, Ekpo M.E. 2011. Absorption approach for recovery and removal of Cd (II) from aqueous solution using coir dust. Arc Appl Sci Res. 3: 402-412.
Fauzi M.T. 2009. Biocontrol ability of Puccinia abrupta var. partheniicola on different growth stages of Parthenium weed (Parthenium hysterophorus L.). HAYATI J Biosci. 16: 83-87.
Gao J, Sun L, Yang X, Liu J.X. 2013. Transcriptomic analysis of cadmium stress response in the heavy metal hyperaccumulator Sedum alfredii Hance. PloS one.  8: e64643.
Gupta A. 1990. Population ecology of Parthenium hysterophorus L., in herbaceous communities of central India (State- Maharashtra). Evolutionary Morphology and Ecology, Academy of Science. USSR 1-36.
Huerta-Cepas J, Szklarczyk D, Forslund K, Cook H, Heller D, et al. 2015. eggNOG 4.5: a hierarchical orthology framework with improved functional annotations for eukaryotic, prokaryotic and viral sequences. Nucleic acids res. 44: 286-93.
Javaid A, Shah M.B. 2008. Use of parthenium weed as green manure for maize and mungbean production. Philippine Agric Sci. 91: 478-82.
Kaur M, Aggarwal N.K, Kumar V, Dhiman R. 2014. Effects and management of Parthenium hysterophorus: A weed of global significance. Int scholarly res notices. 20.
Khan R.A, Ahmed M, Khan M.R, Yasir M, Muhammad B, Khan R. 2011. Nutritional investigation and biological activities of Parthenium hysterophorus. African J Pharmacy Pharmacol. 5: 2073-2078.
Keshwal R.L. 1982. Spread of Parthenium phyllody under field condition. Indian J. Weed Sci. 14: 34-36
Kumar R, Vashisth D, Misra A, Akhtar M.Q, Jalil S.U, Shanker K, Gupta M.M, Rout P.K, Gupta A.K, Shasany A.K. 2016. RNAi down-regulation of cinnamate-4-hydroxylase increases artemisinin biosynthesis in Artemisia annua. Sci rep. 6: 26458.
Kumar S, Chashoo G, Saxena A.K, Pandey A.K. 2013. Parthenium hysterophorus: a probable source of anticancer, antioxidant and anti-HIV agents. BioMed Res Int. 17.
Kushwaha V.B, Maurya S. 2012. Biological utilities of Parthenium hysterophorus. J Appl Natural Sci. 4: 137-43.
Li D, Wang X, Deng Z, Liu H, Yang H, He G. 2016. Transcriptome analyses reveal molecular mechanism underlying tapping panel dryness of rubber tree (Hevea brasiliensis). Sci Rep. 6: 11-13.
Li Z, Zhang L, Che H, Liu H, Chi M, Luo D, et al. 2011. A disease associated with phytoplasma in Parthenium hysterophorus. Phytoparasitica. 39: 407–410. 
Livak K.J, Schmittgen T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods.  25: 402-8.
Lasat M.M. 2000. Phytoextraction of metals from contaminated soil: a review of plant/soil/metal interaction and assessment of pertinent agronomic issues. J Hazard Subst Res. 2: 1-25.
Malik R.N, Husain S.Z, Nazir I. 2010. Heavy metal contamination and accumulation in soil and wild plant species from industrial area of Islamabad, Pakistan. Pak. J. Bot. 42: 291-301.
Misra A, Chanotiya C.S, Gupta M.M, Dwivedi U.N, Shasany A.K. 2012. Characterization of cytochrome P450 monooxygenases isolated from trichome enriched fraction of Artemisia annua L. leaf. Gene. 510: 193-201.
Morton J.F. 1981. The puzzling whitetop. Parthenium hysterophorus: Noxious weed, health hazard, folk-remedy, flea repellent. Unpublished report, Univ. of Miami, Florida.
Narasimhan T.R, Murthy B.K, Rao P.S. 1993 Nutritional evaluation of silage made from the toxic weed Parthenium hysterophorus in animals. Food chem toxicol. 31: 509-15.
Navie S.C, Mcfadyen R.E, Panetta F.D, Adkins S.W. 1996. The biology of Australian weeds. 27. Parthenium hysterophorus L. Plant Prot Quart. 11: 76-88.
Oudhia P. 2001. Parthenium hysterophorus: Traditional Medicinal Uses. Society for Parthenium Management (SOPAM).
Pandey K, Sharma P.K, Dudhe R. 2012. Anticancer Activity of Parthenium hysterophorus Linn and Oldenlandia corymbosa Lam by Srb Method. Sci Rep. 1: 1-3.
Patel S. 2011. Harmful and beneficial aspects of Parthenium hysterophorus: an update. 3 Biotech. 1: 1-9. doi: 10.1007/s13205-011-0007-7
Ponciano G, Mcmahan C.M, Xie W, Lazo G.R, Coffelt T.A, Collins-Silva J, et al . 2012. Transcriptome and gene expression analysis in cold-acclimated guayule (Parthenium argentatum) rubber-producing tissue. Phytochemistry. 79: 57-66.
Radojčićredovniković I, Glivetić T, Delonga K, Vorkapić-Furač J. 2008. Glucosinolates and their potential role in plant. Periodicum biologorum. 110: 297-309.
Saini A, Aggarwal N.K, Sharma A, Kaur M, Yadav A. 2014. Utility potential of Parthenium hysterophorus for its strategic management. Adv Agric. 22.
Sanghamitra K, Prasada Rao P.V, Naidu G.R. 2012. Uptake of Zn (II) by an invasive weed species Parthenium hysterophorus L. Appl Ecol Environ Res. 10: 267-90.
Sanghamitra K, Rao P.P, Naidu G.R. 2011. Heavy metal tolerance of weed species and their accumulations by phytoextraction. Indian Journal of Science and Technbology. 4: 285-90.
Sharma V, Pant D. 2015. Weed Plants for Heavy Metal Management. J Agroecol Natural Resou Manag. 2: 14-17.
Tian S, Gu C, Liu L, Zhu X, Zhao Y, Huang S. 2015. Transcriptome profiling of Louisiana iris root and identification of genes involved in lead-stress response. Int J molecular sci. 16: 28087-97.
Yadav A, Thorat V, Bhale U, Shouche Y. 2015. Association of 16SrII-C and 16SrII-D subgroup phytoplasma strains with witches’ broom disease of Parthenium hysterophorus and insect vector Orosiusal bicinctus in India. Australasian Plant Dis. Notes. 10: 31.
Yang J, Li K, Zheng W, Zhang H, Cao X, Lan Y, Yang C, Li C. 2015. Characterization of early transcriptional responses to cadmium in the root and leaf of Cd-resistant Salix matsudana Koidz. BMC Genomics. 16: 705.
Zhang X, Liu T, Duan M, Song J, Li X. 2016. De novo transcriptome analysis of Sinapis alba in revealing the glucosinolate and phytochelatin pathways. Front plant sci. 7.
Zhang Y, Teoh K.H, Reed D.W, Maes L, Goossens A, Olson DJ, et al. 2008. The molecular cloning of artemisinic aldehyde Δ11 (13) reductase and its role in glandular trichome-dependent biosynthesis of artemisinin in Artemisia annua. J Biol Chem.  283: 21501-8.