ISC, Google Scholar, CAB Abstracts

Document Type : Original Article

Authors

Mycology Research Laboratory, Department of Biological Sciences, R.D. University, Jabalpur, Madhya Pradesh, India.

10.26655/jrweedsci.2019.1.3

Abstract

Microbes and their secondary metabolites offer a benign and eco-friendly alternative to manage weed. Mycoherbicide production for biological control of weeds requires a series steps, from selection of a suitable microbial strain to final formulation. Thus, this study aimed to select potential fungi for production of secondary metabolites with herbicidal activity for control of Parthenium weed. In the present study, phytopathogenic fungi namely Fusarium spp. were isolated from infected tissues of Parthenium and evaluated against Parthenium hysterophorus, a problematic monocotyledonous weed of open lands, Agriculture, Horticulture and Forests. Herbicidal potential of Cell Free Culture Filtrate (CFCF) of three strains of Fusarium spp. coded as FGCCW#16, FGCCW#43 and FGCCW#55, against Parthenium hysterophorus were evaluated by seedling and shoot cut bioassays.  Maximum mortalities of shoots, seedlings and phytotoxic damage were obtained from 21 days old cell free culture filtrate (CFCF) of FGCCW#16 at 100% concentration. Significant reduction in biological contents i.e. photosynthetic pigment and protein was observed in the host weed on treatment with the CFCF as determined by detached leaf bioassay. Phytotoxic damage such as severe wilting, chlorosis, necrosis and complete collapse of the entire parts of the weed were also noticed due to CFCF application.

Keywords

Abbas H.K., Vesonder R.F., Boyette C.D., Peterson S.W. 1992. Phytotoxicity of fumonisin B1 on weed and crop species. Weed Tech. 6: 549-552.
Adkins, S.W., Navie, S.C. and Dhileepan, K.2005. In: Proc. of second International Conference on Parthenium management, UAS, Bangalore, India. (eds Ramchandra Prasad T.V., Nanjappa H.V., R. Devendra, A. Manjunath, Subramanya, Chandrashekhar S. C., Kiran kumar V.V., Jayaram K.A. and Prabhakar Setty T.K.): 11-28.
Amsellem Z., Barghouthi S., Cohen B., Goldwasser Y., Gressel J., Hornok L., Kerenyi Z., Kleifeld Y., Klein O., Kroschel J., Sauerborn J., Muller-Stover D., Thomas H., Vurro M., Zonno, M.C. 2001a. Recent advances in the biocontrol of Orobanche (broomrape) species. Biocontrol. 46: 211-228. 
Amsellem Z., Kleifeld Y., Kerenyi Z., Hornok L., Goldwasser Y., Gressel J. 2001b. Isolation, identification and activity of mycoherbicidal pathogen from juvenile broomrape plants. Biol. Control. 21: 274-284.
Amusa N.A. 2006. Microbially produced phytotoxins and plant disease management. Afr. J. Biotech. 5: 405-414.
Aneja KR. 1991. Deadly weed Parthenium hysterophorus and its control-a review. In: Aery N.C., Chaudary B.L. (eds) Botanical researches in India. Himanshu Publications, Udaipur, India: 258-269
Azam I.E., Abouzeid M.A., Boari A., Vurro M., Evidente A. 2003. Identification of phytotoxic metabolites of a new Fusarium species inhibiting germination of Striga hermonthica seeds. Phytopathol. Mediterr. 42: 65-70.
Bajwa A.A., Chauhan B.S., Farooq M., Shabbir A., Adkins S.W. 2016. What do we really know about alien plant invasion? A review of the invasion mechanism of one of the world's worst weeds. Planta. 244: 39-57.
Boyetchko S.M., Peng G. 2004. Challenges and strategies for development of mycoherbicides. In: Arora DK (Ed) Fungal Biotechnology in Agricultural, Food, and Environmental Applications, Marcel Dekker, New York: 111-121.
Dayan F.E., Owens D.K., Duke S.O. 2012. Rationale for a natural products approach to herbicide discovery. Pest Manag Sci. 68: 519-528.
Deshpande K.S., Deshpande U.K., Rathore O.S. 1982. Biological control of Parthenium hysterophorus L. Indian Botanical Report. 1: 40-42.
Dor E., Evidente A., Amalfitano C., Agrelli D., Hershenhorn J. 2007. The influence of growth conditions on biomass, toxins and pathogenicity of Fusarium oxysporum f. sp. orthoceras, a potential agent for broomrape control. Weed Res. 47: 345-52.
Farkya S., Pandey A.K., Rajak R.C. 1994. Evaluation of Fusarium spp. as Mycoherbicides for Parthenium I: Host Specificity. Nat. Acad. Sci. Letters. 17: 81-82.
Gerwick B.C., Sparks T.C. 2014. Natural products for pest control: an analysis of their role, value and future. Pest Manag Sci. 70: 1169-1185.
Hoagland R.E. 1990. Microbes and Microbial metabolite as herbicides. In: ACS Symp series 439, ACS Washington, D.C., USA.
Joseph S., Lal S., and Pandey A.K.  2002. Preliminary evaluation of herbicidal potential of Streptomyces WC#150 against Lantana camara. Ann Pl Protec. Sci. 10:134-136.   
Kaur M., Aggarwal N.K., Kumar V., Dhiman R. 2014. Effects and management of Parthenium hysterophorus: a weed of global significance. International Scholarly Research Notices, ID 368647: 12.
Kauraw L.P., Chile A., Bhan V.M. 1997. Evaluation of Fusarium pallidoroseum (Cooke) Sacc. for the Biocontrol of Parthenium hysterophorus L. In: Proceedings of First International Conference on Parthenium hysterophorus Management, 6-8 October, Dharwad, India: 70-74.
Kumar C.V., Rao A.S. 1977. Two new leaf spot diseases. Indian Phytopath. 30: 118-120.
Kumar S. 2009. Biological control of Parthenium hysterophorus in India: status and prospects. Indian J Weed Sci. 41: 1-18
Mishra D. 1985. PhD Thesis, Biosciences, Dept of Biological Sciences, R.D. University, Jabalpur.
Pandey A.K., Luka B.M., Hasija S.K., Rajak R.C. 1991. Pathogenicity of some fungi to Parthenium hysterophorus an obnoxious weed in Madhya Pradesh. J Biol Contr. 5: 113-115.
Pandey A.K., Mishra J., Rajak R.C., Hasija S.K. 1996. In: Herbal Medicines, Biodiversity and Conservation Strategies (ed. Rajak R.C., Rai M.K.). International Book distributors, DehraDun: 104-138.
.Pandey A.K., Shrivastava G.M., Singh A.K., Verma S.K. 2003. Herbicidal potential of secondary metabolites of Sclerotium rolfsii against Parthenium: A preliminary observation. J. Basic Appl. Mycol. 2: 27-30.
Pandey A.K., Archana-Pandey G. M., Shrivastava Rajak R.C. 2004a. In: Microbiology and Biotechnology for Sustainable Development (ed. Jain, P.C.), CBS Publishers and Distributors, New Delhi. pp 42-58.  
Pandey A.K., Singh A.K., Quereshi S., Agrawal D. 2004b. Herbicidal activities of secondary metabolites of Streptomyces sp. against Hyptis suaveolens.J. Basic. Appl. Mycol. 3: 95-97.
Rajak R.C., Farkya S., Hasija S.K., Pandey A.K. 1990. Fungi associated with congress weed (Parthenium hysterophorus L.) Proc. Nat. Acad. Sci. India. 60: 165-168.
Rao A.P., Rao A.S. 1987. New fungal diseases of some weeds. Indian Botanical Reporter 6: 38.
Sauerborn J., Müller-Stöver D., Hershenhorn J. 2007. The role of biological control in managing parasitic weeds. Crop Prot. 26: 246-54.
Saxena, S. and Pandey, A.K. 2001. Microbial metabolites as eco-friendly agrochemicals for the next millennium. Appl.Micrbiol.Biotechnol.55: 395-403.
Saxena S., Pandey A.K. 2002. Evaluation of an indigenous isolate of Alternaria alternata LC#508 for use as a mycoherbicide for Lantana camara. Crop Prot. 20: 73-75.
Thapar R., Singh A.K.,  Pandey A.K., Pandey A.K. 2002. Bioactivity of CFCF of Curvularia lunata in Parthenium hysterophorus L. J. Basic Appl. Mycol. 1: 126-129.
Vikrant P., Verma K.K.,  Rajak R.C., Pandey A.K. 2006. Characterization of a phytotoxin from Phoma herbarum for management of Parthenium hysterophorus. J. Phytopathol. 154: 1-8.
Walker H.L., Templeton G.E. 1978. In vitro production of phytotoxic metabolites by Colletotrichum gloeosporioides f sp aeschynomene. Plant Sci Lett. 13: 91-99.
Zonno M.C., Vurro M. 2002. Inhibition of germination of Orobanche ramosa seeds by Fusarium toxins. Phytoparasitica. 30: 519-24.